This paper explores the interaction mechanism between the entrepreneurial motivation, entrepreneurial opportunity recognition and entrepreneurial performance of Chinese female e-commerce platform entrepreneurs. Mature research showed that entrepreneurial performance can be affected by different types of entrepreneurial motivation, entrepreneurial intention, risk propensity, entrepreneurial opportunity recognition and some other variables. Therefore, this paper proposed a research model to explore how different types of entrepreneurial motivation affect entrepreneurial performance by taking entrepreneurial opportunity recognition as a mediator variable. We used the PLS-SEM method to test the proposed model, and collected 200 female entrepreneurs' questionnaire from various regions in China who used different e-commerce platforms to start their businesses. Our findings suggest there are significant effects of developmental and self-actualization motivation on entrepreneurial performance of female entrepreneurs in China who choose online platform to entrepreneurship; Survival and developmental motivation both have a significant effect on entrepreneurial opportunity profitability; Entrepreneurial opportunity recognition, both opportunity profitability and opportunity feasibility, plays a significant effect on entrepreneurial performance; Entrepreneurial opportunity profitability produce a partial mediating effect between developmental motivation and entrepreneurial performance.